SP100 may play a role in the control of gene expression.
Applications
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:1000-1-2000 Western Blot: 1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide corresponding to aa209-227, CETEQINAKRKDTTSDKDD, of human Sp100.
Form
Supplied as a liquid.
Purity
Serum
Specificity
Recognizes human Sp100. Specificity was evaluated by indirect immunoflourescence and western blotting (see Figure 1A and B).
References
1. Condemine et al. (2006) Characterization of endogenous human promyelocytic leukemia isoforms. Cancer Res. 66:6192. 2. Krejcí et al. (2008) Nuclear organization of PML bodies in leukaemic and multiple myeloma cells. Leukemia Research 32(12):1866-1877.