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171872 Anti-HCV1b (R155K), Active, Recombinant (Hepatitis C virus genotype 1b)

Specifications
References
Swiss Prot
O92972
Grade
Highly Purified
Accession #
AF054247
Shipping Temp
Dry Ice
Storage Temp
-70°C

Fusion protein corresponding to the serine protease NS3 (a.a.3-181) and cofactor NS4A (a.a.2132) from Hepatitis C virus genotype 1b (GenBank Accession No. AF054247) with Arg-to-Lys mutation on a.a.155, and Nterminal Flag-His tag, MW= 22.3kD,expressed in an E.coli expression system.

Source
Recombinant protein corresponding to HCV1b at N-terminus, fused to Flag-His tag expressed in E.coli.
Molecular Weight
~22.3kD
Specific Activity
12pmole/min/ug
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Recombinant, E. coli
Purity
~90%
Form
Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 400mM NaCl, 0.2% Triton X-100, 10mM b-mercaptoethanol, 100ug/ml Flag peptide, 30% glycerol
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 400mM NaCl, 0.2% Triton X-100, 10mM b-mercaptoethanol, 100ug/ml Flag peptide, 30% glycerol
Purity
~90%
References
1. Failla, C., et al., J. Virol. 1994 June;68(6):3753-3760. 2. Lang Kuhs, K.A., et al., Mol. Ther. 2012 May;20(3):669-678. 3. Massariol, MJ., et al., Biochem Biophys Res Commun. 2010 Jan 1;391(1):692-697.
USBio References
No references available
Conjugates
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