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172029 Mouse Anti-Actin, Smooth Muscle (ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P62736 (Human)
Isotype
IgG2a,k
Clone Number
SPM332
Grade
Affinity Purified
Applications
E FC IF IHC IP WB
Crossreactivity
Bo Ca Ch Fe Gt Gp Hu Mk Mo Po Rb Rt Sh
Gene ID
59 (Human)
Shipping Temp
Blue Ice
Storage Temp
-20°C
ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46

Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Applications
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1ug/ml Western Blotting: 0.5-1ug/ml Immunoprecipitation: 0.5-1ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. Optimal dilution for a specific application should be determined.
Positive Control
Blood vessels in all tissues, smooth muscle or leiomyosarcoma
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 172029-AF.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes Actin, Smooth Muscle. Species Crossreactivity: Human, Baboon, Monkey, Bovine, Porcine, Sheep, Goat, Feline, Canine, Rabbit, Mouse, Rat, Guinea pig and Chicken.
References
1. Skalli O et. al. Journal of Cell Biology, 1986, 103:2787-96.
USBio References
No references available
Conjugates
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