USBio Logo

172102-AF-PE Mouse Anti-gp100 (95kD melanocyte-specific Secreted GlycoProtein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte Protein Pmel 17, Melanoma Associated ME20 antigen, Melanosomal matrix Protein17, p100, p26, PMEL17, Premelanosome Protein, Secreted melanoma-Associated ME20 antigen, SILV, Silver Homolog, Melanosome) (PE)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P40967 (Human)
Conjugate
PE
Isotype
IgG2b,k
Clone Number
SPM286
Grade
Affinity Purified
Applications
FC FLISA IF IHC
Crossreactivity
Eq Hu
Gene ID
6490 (Human)
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
95kD melanocyte-specific Secreted GlycoProtein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte Protein Pmel 17, Melanoma Associated ME20 antigen, Melanosomal matrix Protein17, p100, p26, PMEL17, Premelanosome Protein, Secreted melanoma-Associated ME20 antigen, SILV, Silver Homolog, Melanosome

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Applications
Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
Positive Control
SK-MEL-28 cells or Melanoma
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Membranes from a human melanoma metastasis. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes gp100. Species Crossreactivity: Human and Equine. Does not react with rat.
References
1. Vennegoor C. et al., Am.J. Pathol. 130: 179-192, 1988.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved