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172141 Mouse Anti-Plasma Cell Marker (Plasma B cell, Plasmocytes)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a
Clone Number
SPM310
Grade
Affinity Purified
Applications
IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Plasma B cell, Plasmocytes

It recognizes an intra-cytoplasmic antigen, which shows a very high degree of specificity for plasma cells. This antigen is present in normal as well as neoplastic plasma cells. Plasma cells, which are large lymphocytes derived from an antigen-specific B cell, secrete antibodies and are responsible for humoral immunity. Plasma cells differentiate from B cells upon stimulation by CD4+ lymphocytes. The B cell acts as an antigen-presenting cell (APC), consuming an offending pathogen, which is taken up by the B cell by phagocytosis and broken down within proteosomes. Plasma cells contain basophilic cytoplasm; their nucleus contains heterochromatin organized in a characteristic cartwheel arrangement. This MAb superbly recognizes normal and neoplastic plasma cells in routine formalin-fixed, paraffin-embedded tissue sections. It is of potential value in identifying myeloma or plasmacytoma in bone marrow or other tissues. It also helps differentiate lympho-plasmacytoid lymphoma from lymphocytic and follicular lymphoma. Note that this MAb is not suitable for staining frozen tissues.

Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT [Staining of formalin-fixed tissues is ENHANCED by digestion with trypsin (or Protease XXV from Sigma) at 1mg/ml PBS for 5 min at 37°C] Optimal dilution for a specific application should be determined.
Positive Control
Tonsil or lymph node
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Pancreatic cancer-related mucin. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 172141-AF.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human Plasma Cell Marker. Does not react with rat.
References
1. Turley H et. al. Journal of Clinical Pathology, 1994, 47(5):418-22.
USBio References
No references available
Conjugates
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