ASC

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Isotype

IgG

Clone Number

AL177

Grade

Affinity Purified

Applications

IC IHC IP WB

Crossreactivity

Hu Mo

Shipping Temp

Blue Ice

Storage Temp

-20°C

Notes

Preservative Free

ASC promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. It is a component of the inflammasome, a protein complex which also includes NLRP3/NALP3, CARD8 and CASP1 and whose function is the activation of proinflammatory caspases.

Applications

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions

Western Blot: 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:500 Immunocytochemistry: 1:200 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to amino acids at the N-Terminal of human ASC

Form

Supplied as a liquid in PBS, 10% glycerol. No preservative added.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human ASC. Species Crossreactivity: mouse

References

1. The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta: F. Martinon, et al.; Mol. Cell. 10: 417 (2002). 2. NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflamma- tory disorder: L. Agostini, et al.; Immunity 20: 319 (2004). 3. P2X7 Receptor Differentially Couples to Distinct Release Pathways for IL-1beta in Mouse Macrophage: P. Pelegrin, et al.; J. Immunol. 180: 7147 (2008). 4. Inflammatory role of ASC in antigen-induced arthritis is independent of caspase-1, NALP-3, and IPAF: L. Kolly, et al.; J. Immunol. 183: 4003 (2009). 5. Activation of autophagy by inflammatory signals limits IL-1b production by targeting ubiquitinated inflamma- somes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13: 255 (2012). 6. NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T. Heneka, et al.; Nature 493: 674 (2013).

USBio References

No references available

Product	Size	List	Your Price	Qty	Ext Price
Subtotal:	Subtotal:
Subtotal:	Subtotal:
Total Coupon Savings:	Total Coupon Savings:	()
Your cart is currently empty.
- Coupon Code

Product

Size

List

Your Price

Qty

Ext Price

Subtotal:

Total Coupon Savings:

()

Your cart is currently empty.

- Coupon Code

207062 Rabbit Anti-ASC (Pycard, Target of Methylation-induced Silencing 1, TMS1, Caspase Recruitment Domain-containing Protein 5, Apoptosis-associated Speck-like Protein Containing CARD, CARD5)

Clone Type

Host

Source

Isotype

Clone Number

Grade

Applications

Crossreactivity

Shipping Temp

Storage Temp

Notes

References

USBio References

Conjugates

Pricing

Order

Cart Summary

Recently Viewed

Special Offers

Contact Us

Site Map

Technical Resources

Distributors

Payment Methods