This intronless gene encodes a 70kD heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Applications
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:500 Western Blot: 1:1000 Immunoprecipitation: 1ug Optimal dilutions to be determined by the researcher.
Extinction Coefficient: 90,000
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. ATTO 488 conjugates are sensitive to light.
Immunogen
Recombinant protein corresponding to human HSPA1A.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol. Labeled with ATTO 488.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human HSPA1A. Detects several members of the heat shock protein 70kD gene family including Hsp70, Hsc70, Grp78 and following heat shock, Hsp72. Species Crossreactivity: amphibia, chicken, fish, drosophila, mouse, rat, yeast.
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