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208015 Goat Anti-ATP5A1 (ATP synthase, H+ transporting, Mitochondrial F1 complex, alpha subunit 1, Cardiac muscle, ATP5A, ATP5AL2, ATPM, MOM2, OMR, ORM, hATP1, ATP synthase alpha chain, Mitochondrial, ATP synthase subunit alpha, mitochondrial, ATP synthase, H+ transportin)

Specifications
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo Rt
Accession #
NP_004037.1; NP_001244263.1; NP_001001935.1
Gene ID
498, 11946 (mouse), 65262 (rat)
Gene #
ATP5A1
Shipping Temp
Blue Ice
Storage Temp
-20°C

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites By similarity.

Applications
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:128,000 Western Blot: 0.01-0.03ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-RVHGLRNVQAEE, from the internal region (near N-terminus) of the protein sequence according to NP_004037.1; NP_001244263.1; NP_001001935.1. Species sequence homology: canine, porcine and bovine
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes ATP5A1. This antibody is expected to recognize all reported isoforms (NP_004037.1; NP_001244263.1; NP_001001935.1). Reported variants represent identical protein: NP_001244264.1, NP_001001935.1. Species Crossreactivity: human, mouse and rat
Conjugates
Pricing
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