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214686-ML650 Mouse Anti-CD40 (MaxLight 650)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Bovine
Swiss Prot
Q28203
Conjugate
MaxLight™650
Isotype
IgG1
Clone Number
IL-A156
Grade
Affinity Purified
Applications
FC
Crossreactivity
Bo Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

CD40 cell surface antigen is expressed by B lymphocytes and a subset of T lymphocytes. CD40 plays an important role in the differentiation of B lymphocytes into effector cells, and is also involved in interactions between T and B lymphocytes.
Applications
Suitable for use in Flow Cytometry and Functional Assays. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes bovine CD40. Species Crossreactivity: sheep
References
1. Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70interaction with different bovine antigen-presenting cells. Scand J Immunol. 61: 242-50. 2. Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP subset in lymph dendritic cells from the upper aero-digestive tract. J. Leukoc. Biol. 76: 553-561. 3. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells. J. Gen. Virol. 84: 1771-1780. 4. Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro. Immunology 108: 55- 61. 5. Haas, K.M. et al. (2001) Enhancing effects of anti-CD40 treatment on the immune response of SCID-bovine mice to Trypanosoma congolense infection. J Leukoc Biol. 70: 931-40.
USBio References
No references available
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