Equine CD8 is expressed by a subset of T lymphocytes.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap..
Note: Applications are based on unconjugated antibody.
Immunogen
Equine peripheral blood mononuclear cells (PBMC)
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes equine CD8.
References
1. Ferreira-Dias, G. et al. (2005) Seasonal reproduction in the mare: possible role of plasma leptin, body weight and immune status. Domest Anim Endocrinol. 29: 203-13. 2. Krakowski, L. et al. (2017) Changes in blood lymphocyte subpopulations and expression of MHC-II molecules in wild mares before and after parturition J Vet Res. 61 (2): 217-21.