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214735 Rat Anti-CD8

Specifications
References
Clone Type
Monoclonal
Host
Rat
Source
Canine
Swiss Prot
P33706
Isotype
IgG1
Clone Number
YCATE55.9
Grade
Affinity Purified
Applications
FC IF IHC
Crossreactivity
Ca
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD8 was clustered as Canine CD8 in the First Canine Leukocyte Antigen Workshop (Cobbold et al. 1994). Reacts with a rat cell line transfected with cDNA for canine CD8a (Gorman et al. 1994) and blocks MHC class I dependant T-cell responses in vitro and in vivo.

Applications
Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:50-1:100. Use 10ul to label 1x10e6 cells in 100ul. Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Canine CD8 alpha chimeric human IgG1 Fc fusion protein.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes canine CD8.
References
1. Cobbold, S and Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 43: 137-154. 2. Gorman, S. et al. (1994) Isolation and expression of cDNA encoding the canine CD4 and CD8a antigens. Tissue Antigens. 43:184-188. 3. Watson, C. J. E. et al. (1993) CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog. Brit. J. Surg. 80: 1389-1392. 4. Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.Can J Vet Res. 73: 137-143. 5. Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.J Nutr. 133: 1158-62. 6. Bird, R.C. et al. (2010) An autologous dendritic cell canine mammary tumor hybrid-cell fusion vaccine.Cancer Immunol Immunother. 60: 87-97. 7. Bund, D. et al. (2010) Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies.Cell Immunol. 263: 88-98. 8. Estrela-Lima, A. et al. (2010) Immunophenotypic features of tumor infiltrating lymphocytes from mammary carcinomas in female dogs associated with prognostic factors and survival rates.BMC Cancer. 10: 256. 9. Huang, Y.C. et al. (2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.J Leukoc Biol. 84: 1501-10. 10. Kornegay, J.N. et al. (2010) Widespread muscle expression of an AAV9 human mini-dystrophin vector after intravenous injection in neonatal dystrophin-deficient dogs.Mol Ther. 18: 1501-8. 11. Pichavant, C. et al. (2010) Expression of dog microdystrophin in mouse and dog muscles by gene therapy.Mol Ther. 18: 1002-9. 12. Pinheiro, D. et al (2011) Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog.Immunology. 132: 111-22. 12. Reis, A.B. et al. (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.Clin Exp Immunol. 146: 303-11.
USBio References
No references available
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