I-TAC is a member of the CXC chemokine family, which signals through binding to CD183 (CXCR3), and is regulated by interferon gamma and beta. I-TAC is highly expressed by peripheral blood leucocytes, the pancreas and liver, and acts as a potent chemoattractant for IL-2 activated T cells, as well as a natural antagonist for CD195 (CCR5).
Applications
 Suitable for use in ELISA, Western Blot, Immunohistochemistry, Functional Assays. Other applications not tested.
Recommended Dilution
 ELISA: 0.5-2ug/ml Western Blot: 0.1-0.2ug/ml Immunohistochemistry: Paraffin. Antigen retrival using heat treatment prior to staining of paraffin sections is required. Functional Assays: 7-8ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
 Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human I-TAC.
Form
Supplied as a lyophilized powder in PBS. No preservative added. Reconstitute with 10ul sterile ddH2O. For long term storage the addition of 0.09% sodium azide is recommended.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human I-TAC.