tRNA-guanine transglycosylase (TGT; EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single protein of 43 kD. In contrast, mammalian TGT appears to be a heterodimer consisting of a 60-kD subunit (USP14; MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and Katze, 2001 [PubMed 11255023]).
Applications
Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:500-1:3000 Immunohistochemistry (Paraffin): 1:100-1:1000 Immunocytochemistry/Immunofluorescence: 1:100-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein fragment corresponding to the central region from human QTRT1. Species sequence homology: xenopus (tropicalis and laevis) and zebrafish
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.0, 0.01% Thimerosal, 20% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human QTRT1. Species Crossreactivity: mouse.
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