CXCR5, a Chemokine Receptor, also called Burkitt's lymphoma receptor 1, is required for normal development of Peyer's patches, inguinal lymph nodes, and splenic follicles. CXCR5 binds B-lymphocyte chemoattractant (BLC) and is involved in B-cell homing to follicles in lymph nodes and spleen. Two isoforms are produced by alternative splicing.
Applications
Suitable for use in Flow Cytometry, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50-200 Western Blot: 1:500-1000 Immunohistochemistry: 1:20-100 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human CXCR5
Form
Supplied as a lyophilized powder in PBS. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from culture supernatant.
Specificity
Recognizes human CXCR5.