LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant protein corresponding to mouse LYVE-1.

Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.

Purified by Protein G affinity chromatography from culture supernatant.

Recognizes mouse LYVE-1 in western blot. Reactivity to LYVE-1 of other species is not tested.