Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Recombinant protein corresponding to human Gia-2.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.

Purified.

Recognizes human Gia-2. Species sequence homology: bovine (100%), mouse (69%), rat (69%), human (62%) and guinea pig (43%). Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.