PDE9A is a ubiquitous protein belonging to the cyclic nucleotide phosphodiesterase family which regulate the signaling of cyclic nucleotides such as cGMP and cAMP. PDE9A has a higher affinity for cGMP. It contains a catalytic site that associates with cofactors and hydrolyzes cGMP thereby terminating cGMP signaling for a number of cellular processes. PDE9A has been indirectly linked to signaling pathways involved in cognitive processes. Previous studies suggested that inhibition of PDE9A enhanced long-term potentiation (a widely studied mechanism of learning and memory); PDE9A may also effect changes in the plasticity of hippocampal neurons which are also important for certain types of cognitive processes. In addition to the brain, PDE9A is also highly expressed in the spleen, prostate, colon, kidney, and heart and may be involved in a range of physiological processes in these systems, via cGMP-amplified signaling.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Immunohistochemistry: 1:100 Optimal dilutions to be determined by the researcher.

Human spleen tissue lysate

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Linear peptide corresponding to human PDE9A, conjugated to KLH. Species sequence homology: rat and chimpanzee (100%)

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography.

Recognizes human PDE9A. Species Crossreactivity: mouse and rhesus macaque.