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227416-ML490 Mouse Anti-CD39 (MaxLight 490)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P49961
Conjugate
MaxLight™490
Isotype
IgG1
Clone Number
A1
Grade
Affinity Purified
Applications
FC IF
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Recognizes the human CD39 cell surface antigen, a ~70-100kD molecule expressed on peripheral blood B cells, monocytes and T lymphocytes, and weakly by granulocytes.
CD39 has intrinsic ecto-ATPase activity (Wang et al. 1996), and expression can be induced on T cells and increased on B cells, as a late activation antigen (Maliszewski et al. 1994).
Mouse anti human CD39, has been shown to block MHC independent target cell recognition by hapten-specific CTL (Scholzen et al. 2009).
Applications
Suitable for use in Immunofluorescence and Flow Cytometry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
PHA activated human lymphocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human CD39.
References
1. Aversa, G.G. et al. (1988) Detection of a late lymphocyte activation marker by A1, A new monoclonal antibody. Transpl. Proc. 20: 49-52. 2. Waugh, J.A. et al. (1989) Staining of normal and rejecting kidney using the activation panel. In: Leucocyte Typing IV. White cell differentiation antigens. Edited by Knapp, W. et al.Oxford University Press. p485. 3. Aversa, G.G. and Hall, B.M. (1989) Activation panel antigen expression on PBL activated by PHA or in MLR. In: Leucocyte Typing IV. White cell differentiation antigens. Edited by Knapp, W. et al. Oxford University Press, p.498. 4. Aversa, G.G. et al. (1989) Use of monoclonal antibodies to study in vivo and in vitro activated lymphocytes. Transpl. Proc. 21: 349-350. 5. Stein, H. et al. (1989) Activated Section report. In: Leucocyte Typing IV. White cell differentiation antigens. Edited by Knapp, W. et al. Oxford University Press, p.387. 6. Suranyi, M.G. et al. (1991) Lymphocyte adhesion molecules in T cell mediated lysis of kidney cells. Kidney International. 39: 312-319. 7. Stockl, J. et al. (2001) Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1 restricted, hapten-specific CTL. J. Immunol. 167: 2724-2733. 8. Scholzen, A. et al. (2009) Plasmodium falciparum-mediated induction of human CD25Foxp3 CD4 T cells is independent of direct TCR stimulation and requires IL-2, IL-10 and TGFbeta. PLoS Pathog. 5: e1000543. 9. Borsellino, G. et al. (2007) Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression Blood. 110:1225-32. 10. Mittag, D. et al. (2010) The effector T cell response to ryegrass pollen is counterregulated by simultaneous induction of regulatory T cells. J Immunol. 184: 4708-16. 11. Loeuillet, C. et al. (2008) In vitro whole-genome analysis identifies a susceptibility locus for HIV-1. PLoS Biol. 6: e32. 12. Rawstron, A.C. et al. (2010) Chronic lymphocytic leukaemia (CLL) and CLL-type monoclonal B-cell lymphocytosis (MBL) show differential expression of molecules involved in lymphoid tissue homing. Cytometry B Clin Cytom. 78 Suppl 1: S42-6. 13. Alam, M.S. et al. (2009) CD73 is expressed by human regulatory T helper cells and suppresses proinflammatory cytokine production and Helicobacter felis-induced gastritis in mice. J Infect Dis. 199: 494-504. 14. Moreno-Fernandez, M.E. et al. (2011) Regulatory T cells control HIV replication in activated T cells through a cAMP-dependent mechanism. Blood. 117: 5372-80. 15. Guevara-Flores, A. et al. (2008) 5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP- diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci. Mol Biochem Parasitol. 162: 123-33. 16. Glenn, J.R. et al. (2008) Raised levels of CD39 in leucocytosis result in marked inhibition of ADP-induced platelet aggregation via rapid ADP hydrolysis. Platelets. 19: 59-69. 17. Häusler, S.F.M. et al. (2014) Anti-CD39 and anti-CD73 antibodies A1 and 7G2 improve targeted therapy in ovarian cancer by blocking adenosine-dependent immune evasion. Am J Transl Res 6: 129-139.
USBio References
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