CD4 is a 58kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes. As in humans, equine CD4 expression is mutually exclusive with CD8 expression on mature T-cells.

A study undertaken using 227417, to identify CD4 on several wild african equid species, indicates that CD4 recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).

Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.

X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Equine Thymocytes

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography form tissue culture supernatant.

Recognizes equine CD4.