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227427-ML490 Mouse Anti-CD4a (MaxLight 490)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Porcine
Conjugate
MaxLight™490
Isotype
IgG2b
Clone Number
MIL17
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Po
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Recognizes a 55kD porcine homologue to the human CD4 antigen found on the surface of helper-T cells. MIL-17 was confirmed as a member of the CD4a cluster at the ‘Third International Workshop on Swine Leukocyte Differentiation Antigens’ (Haverson et al. 2001). Porcine CD4 is a type 1 trans-membrane member of the immunoglobulin superfamily. Pigs appear unusual amongst mammalian species as they appear to have four populations of resting T lymphocytes. In addition to the two populations of mutually exclusive CD4+/CD8- and CD4-/CD8+ lymphocytes, they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but this is not the case for mature T lymphocytic calls (Saalmuller et al. 1987)
Stains a population of cells with characteristic lymphocyte morphology in immunohistochemistry (Inman et al. 2010).
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry: The epitope recognized is reported to be sensitive to formaldehyde fixation and tissue processing. Acetone fixation is recommeneded for frozen sections. Paraffin sections are not recommeneded. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Leucocytes isolated from porcine gut lamina propria.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes porcine CD4a.
References
1. Saalmüller, A. et al. (2001) Summary of workshop findings for porcine T-lymphocyte - specific monoclonal antibodies. Vet. Immunol. Immunopathol. 80: 35-52. 2. Castellano, G. et al. (2010) Therapeutic targeting of classical and lectin pathways of complement protects from ischemia-reperfusion-induced renal damage. Am J Pathol. 176: 1648-59. 3. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa. J Leukoc Biol. Jun 15. [Epub ahead of print] 4. Kick, A.R. et al. (2010) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae. Res Vet Sci. Dec 11. [Epub ahead of print] 5. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs. J Anim Sci. 90: 649-56. 6. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys. Kidney Int. 58: 838-50. 7. Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro. Cell Biol Int. 2012 Sep 13. [Epub ahead of print] 8. Tuchscherer, M. et al. (2012) Effects of inadequate maternal dietary protein:carbohydrate ratios during pregnancy on offspring immunity in pigs. BMC Veterinary Research 8: 232. 9. Cao, D. et al. (2010) Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of CpG-ODN in newborn piglets. Vaccine. 28: 6006-13. 10. Clapperton, M. et al. (2005) Associations of weight gain and food intake with leukocyte sub-sets in Large White pigs Livestock Production Science 96: 249-60. 11. Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs. Vet Immunol Immunopathol. 104: 131-44. 12. Clapperton, M. et al. (2008) Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance. Animal. 2: 1575-84. 13. Faure, J.P. et al. (2002) Polyethylene glycol reduces early and long-term cold ischemia- reperfusion and renal medulla injury. J Pharmacol Exp Ther. 2002 Sep;302(3):861-70. 14. Faure, J.P. et al. (2004) Evidence for protective roles of polyethylene glycol plus high sodium solution and trimetazidine against consequences of renal medulla ischaemia during cold preservation and reperfusion in a pig kidney model. Nephrol Dial Transplant. 19: 1742-51. 15. Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys. Kidney Int. 62: 654-67. 16. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs. J Anim Sci. 90: 649-56. 17. Langerhuus, S.N. et al. (2010) Brief report: biomarkers of aortic vascular prosthetic graft infection in a porcine model with Staphylococcus aureus. Eur J Clin Microbiol Infect Dis. 29: 1453-6. 18. Lu, X. et al. (2012) Genome-wide association study for T lymphocyte subpopulations in swine. BMC Genomics. 13: 488. 19. Monroy-Salazar, H.G. et al. (2012) Effects of a live yeast dietary supplement on fecal coliform counts and on peripheral blood CD4+ and CD8+ lymphocyte subpopulations in nursery pigs. J Swine Health Prod 20: 276-282. 20. Shi, K. et al. (2008) Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. Vet Microbiol. 129: 367-77. 21. Spreeuwenberg, M.A. et al. (2001) Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning. J Nutr. 131: 1520-7. 22. Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro. Cell Biol Int. 36: 1233-8. 23. Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining. J Immunol Methods. 327: 18-29. 24. Kvist, P.H. et al. (2010) Effect of subcutaneous glucose sensor implantation on skin mRNA expression in pigs. Diabetes Technol Ther. 12: 791-9. 25. Lefevre, E.A. et al. (2012) Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes. PLoS One. 7(3): e32400. 26. Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen- presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire. PLoS One. 7(3): e33707.
USBio References
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