FPGT

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Conjugate

FITC

Isotype

IgG

Grade

Affinity Purified

Applications

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Catalyzes the formation of GDP-L-fucose from GTP and L-fucose-1-phosphate. Functions as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids.

Applications

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Immunogen

Recombinant protein corresponding to human Fucose-1-phosphate guanylyltransferase.

Form

Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).

Purity

Purified by Protein G affinity chromatography.

Specificity

Recognizes human Fucose-1-phosphate guanylyltransferase.

References

1. GDP-L-fucose pyrophosphorylase: purification, cDNA cloning, and properties of the enzyme.Pastuszak I., Ketchum C., Hermanson G., Sjoberg E.J., Drake R., Elbein A.D.J. Biol. Chem. 273:30165-30174(1998).