Immediate PCR amplification of known genes Comparison of gene expression patterns between biological samples Gene mutation analysis Gene cloning and target sequencing

This First Strand cDNA Synthesis BioGenomics™ Kit provides all necessary components to perform 100 first strand cDNA synthesis reactions from total RNA or poly(A) RNA. The system uses Moloney Murine Leukemia Virus Reverse Transcriptase with weak activity of RNase H that allows the synthesis of full-length cDNA from long templates. Oligo(dT)18 and random hexamer primers are provided. Specific primers can also be used with the kit.

The cDNA synthesized using this system can be used directly in PCR amplification. The kit is optimized for maximum yield of full-length cDNA. The addition of ribonuclease inhibitor lowers the risk of mRNA degradation during the reaction.

All kit components are DNase-, RNase-, and protease-free. Each component has been tested for purity and efficacy in first strand cDNA synthesis.

MMLV reverse transcriptase, 100unit/ul, 1x100ul Reaction Buffer, 5x, 1x1ml Oligo(dT)18, 0.5ug/ul, 1x100ul Random Hexamers, 0.2ug/ul, 1x100ul RNasein, 10units/ul, 1x100ul dNTP, 10mM each, 1x250ul Human Placenta total RNA, 1.25ug/ul, 1x10ul Upstream Primer, 25um, 1x50ul Downstream Primer, 25um, 1x50ul DEPC H2O, 1x1.25ml

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.