Can be used for capture of SEB in an ELISA sandwich assay and Western blot detection. It is not recommended for direct detection in an ELISA.
Applications
Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.
Recommended Dilution
ELISA: 1ug/ml Western Blot: 1ug/ml Neutralization: 0.1ng/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Staphylococcal enterotoxin B
Form
Supplied as a liquid in PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes Staphylococcal enterotoxin B. Does not appear to cross react with Staphylococcal enterotoxin C (SEC1).