CD19 is a 95kD cell surface glycoprotein expressed upon cells of the B cell lineage and follicular dendritic cells. CD19 is absent on plasma cells.
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000 ELISA: 1:20,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic non-phosphopeptide mapping around the Y531 phosphorylation site of human CD19.
Form
Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CD19. Species Crossreactivity: monkey and mouse