PTPRC, CD45, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD antigen CD45
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
Applications
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunofluorescence (circulating tumor cells): 1:200-1:1000 ELISA: 1:5000 Immunohistochemistry (circulating tumor cells): 1:100-1:300 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic non-phosphopeptide mapping around the S1007 phosphorylation site of human CD45.
Form
Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CD45. Species Crossreactivity: mouse and rat.
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NCBI
UniProt
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