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311128 Rabbit Anti-Caspase 3, cleaved (Asp175) (Apopain precursor, CASP-3, CPP-32, CPP32, Caspase-3, Cysteine protease CPP32, ICE3, SCA-1, SREBP cleavage activity 1, Yama protein, p17)

Specifications
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P42574
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175. Species Crossreactivity: human, mouse and rat
Conjugates
Pricing
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