G-26 T-lymphocyte-secreted protein, Lymphocyte activation gene 1 protein, LAG-1, MIP-1-beta1-69
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. {ECO:0000269 PubMed:10540332, ECO:0000269 PubMed:12070155, ECO:0000269 PubMed:8525373}.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0ng/ml, corresponding to a specific activity of ＞ 5.0x10e5 IU/mg.
Sequence
APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN
PubMed ID
2462251; 2521882; 2521353; 2247088; 2568930; 1894635; 15843566; 16625196; 15489334; 2809212; 9521068; 8525373; 10540332; 12070155; 12401480; 8134838
Gene Name
CCL4,LAG1,MIP1B,SCYA4
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥97% as determined by SDS-PAGE and HPLC.
Form
Lyophilized from 0.2um filtered PBS, pH 7.4
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Lyophilized from 0.2um filtered PBS, pH 7.4
Purity
≥97% as determined by SDS-PAGE and HPLC.
TDS
NCBI
UniProt
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