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349675 Anti-AMD1, Recombinant, Human, aa68-334, His-Tag (S-adenosylmethionine decarboxylase proenzyme)

Specifications
References
Grade
Purified
Accession #
NP_001625
Shipping Temp
Blue Ice
Storage Temp
-20°C

AMD1, also known as adenosylmethionine decarboxylase proenzyme, is synthesized initially as an inactive proenzyme. The post-translation cleavage follows an unusual pathway, termed non- hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.

Source
Recombinant protein corresponding to aa68-334 of human AMD1, fused to His-tag at N-terminal expressed in E. coli.
Molecular Weight
~33.4kD
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSSMFV SKRRFILKTC GTTLLLKALV PLLKLARDYS GFDSIQSFFY SRKNFMKPSH QGYPHRNFQE EIEFLNAIFP NGAAYCMGRM NSDCWYLYTL DFPESRVISQ PDQTLEILMS ELDPAVMDQF YMKDGVTAKD VTRESGIRDL IPGSVIDATM FNPCGYSMNG MKSDGTYWTI HITPEPEFSY VSFETNLSQT SYDDLIRKVV EVFKPGKFVT TLFVNQSSKC RTVLASPQKI EGFKRLDCQS AMFNDYNFVF TSFAKKQQQQ QS
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
~80% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 20% glycerol.
Purity
~80% (SDS-PAGE)
References
1. Tolbert W.D., et al. (2001) Biochemistry. 40:9484-9494, 2. Xiong H., et al. (1999) J. Biol. Chem. 274:35059-35066.
USBio References
No references available
Conjugates
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