Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Immunohistochemistry: frozen and paraffin Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Synthetic peptide corresponding to aa408-439 from the C-terminal of human c-myc.

Supplied as a liquid in PBS, pH 7.2, 0.02% Proclin-300.

Purified by Immobilized Metal Affinity Chromatography

Recognizes the epitope sequence, EQKLISEEDL, located in aa410-419 of human c-myc protein.