His-Tag, CT

Clone Type

Monoclonal

Host

Rat

Isotype

IgG1,k

Clone Number

3D5 (recomb)

Grade

Affinity Purified

Applications

E WB

Shipping Temp

Blue Ice

Storage Temp

-20°C

Recombinant monoclonal antibody to His tag (C-terminal) with variable regions (i.e., specificity) from the hybridoma 3D5. This is a chimeric rat antibody, based on the original mouse IgG2b format. Manufactured using a proprietary recombinant platform technology.

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Purified His-tagged Fab fragment.

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% Proclin-300.

Purity

Purified by Protein A affinity chromatography.

Specificity

Recognizes Carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins.

References

Original Clone First Published in:|Lindner P, Bauer K, Krebber A, Nieba L, Kremmer E, Krebber C, Honegger A, Klinger B, Mocikat R, Plückthun A. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques. 1997 Jan; 22(1):140-9. PMID:8994661.

USBio References

No references available

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361650 Rat Anti-His-Tag, CT (Polyhistidine Tag) (Chimeric)

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