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361681 Mouse Anti-Mouse IgG2a Isotype Control (Unknown Specificity)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Isotype
IgG2a,k
Clone Number
MOPC-21 (recomb)
Grade
Affinity Purified
Applications
FC WB
Shipping Temp
Blue Ice
Storage Temp
-20°C

Recombinant monoclonal antibody of unknown specificity (isotype control) with variable regions (i.e., specificity) from the hybridoma MOPC-21. This is a re-formatted antibody created as part of a panel offering of antibodies of the same clone in different formats (species, isotype, subtype and modified versions) for use as isotype controls. Manufactured using a proprietary recombinant platform technology.

Applications
Suitable for use in Flow Cytometry, Western Blot and as a Negative Isotype Control. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% Proclin-300.
Purity
Purified by Protein A affinity chromatography.
Specificity
This is an antibody of unknown specificity that can be used as a negative isotype control.
References
1. Bothwell AL et al (VH); Hamlyn PH et al (VL) Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a gamma 2a variable region. (VH), Complete sequence of an immunoglobulin mRNA using specific priming and the dideoxynucleotide method of RNA seq. (VL) Cell. 1981 Jun; 24(3):625-37. (VH), Nucleic Acids Res. 1981 Sep 25; 9(18):4485-94. (VL) PMID:6788376 (VH); 6170937 (VL).
USBio References
No references available
Conjugates
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