RpiA, Recombinant, E. coli, aa1-219, GST-Tag

Swiss Prot

P0A7Z0

Grade

Purified

Shipping Temp

Blue Ice

Storage Temp

-20°C

Phosphoriboisomerase A

Involved in the first step of the non-oxidative branch of the pentose phosphate pathway. It catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate. Can also act on D-ribose-5-diphosphate and D-ribose-5-triphosphate as substrate.

Source

Recombinant protein corresponding to aa1-219 from E. coli rpiA, fused to GST-Tag at N-terminal, expressed in E. coli.

Molecular Weight

~49.9kD

Amino Acid Sequence

MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKGQIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTREKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGVVTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIVK

Storage and Stability

Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Source

Recombinant, E. coli

Purity

≥90% (SDS-PAGE)

Form

Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.

Purity

≥90% (SDS-PAGE)

References

1. Structure of Escherichia coli ribose-5-phosphate isomerase a ubiquitous enzyme of the pentose phosphate pathway and the Calvin cycle.Zhang R.G., Andersson C.E., Savchenko A., Skarina T., Evdokimova E., Beasley S., Arrowsmith C.H., Edwards A.M., Joachimiak A., Mowbray S.L.Structure 11:31-42(2003).

USBio References

No references available

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375093 Anti-RpiA, Recombinant, E. coli, aa1-219, GST-Tag (Ribose-5-phosphate Isomerase A)

Swiss Prot

Grade

Shipping Temp

Storage Temp

Phosphoriboisomerase A

References

USBio References

Conjugates

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