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381636 Mouse Anti-IL8 (AMCF 1, AMCF1, b ENAP, Beta Thromboglobulin like Protein, Beta Thromboglobulin like Protein, C-X-C Motif Chemokine 8, CXC Chemokine ligand 8, CXCL 8, CXCL8, Emoctakin, GCP 1, GCP-1, GCP/IL-8 Protein I, GCP/IL-8 Protein II, GCP/IL-8 Protein III, Inteleukin 8, Interleukin8, K 60, K60, LECT, LUCT, Lymphocyte Derived Neutrophil Activating Factor, Lymphocyte-Derived Neutrophil-Activating Factor, LYNAP, MDNCF, MDNCF-b, MDNCF-c, MONAP, Monocyte Derived Neutrophil Activating Peptide, Monocyte Derived Neutrophil Activating Protein, Monocyte Derived Neutrophil Chemotactic Factor, Monocyte-Derived Neutrophil Chemotactic Factor, Monocyte-Derived Neutrophil-Activating Peptide, NAF, NAP 1, NAP-1, NAP1, Neutrophil Activating Factor, Neutrophil Activating Peptide 1, Neutrophil Activating Protein 1, Neutrophil-Activating Factor, Neutrophil-Activating Protein 1, Protein 3 10C, Protein 3-10C, SCYB 8, SCYB8, Small Inducible cytokine Subfamily B Member 8, T Cell Chemotactic Factor, T-Cell Chemotactic Factor, TSG 1, TSG1)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P10145
Isotype
IgG2b
Clone Number
17J74
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Gene ID
3576
Gene #
CXCL8
Shipping Temp
Blue Ice
Storage Temp
-20°C

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to IL8.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes IL8. Species Crossreactivity: human
References
1. Schmid J.,et al.J. Immunol. 139:250-256(1987). 2. Matsushima K.,et al.J. Exp. Med. 167:1883-1893(1988). 3. Mukaida N.,et al.J. Immunol. 143:1366-1371 (1989). 4. Kowalski J.,et al.Mol. Cell. Biol. 9:1946-1957(1989). 5. Hotta K.,et al.Immunol. Lett. 24:165-169(1990).
USBio References
No references available
Conjugates
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