Hes1 is the transcriptional repressor of genes that require a bHLH protein for their transcription. Hes1 may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Hes1 binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
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