CRISPR/Cas9
The Mutation Detection Kit provides a simple, reliable, and rapid method for the detection of site specific cleavage of genomic DNA that is extracted from cells transfected with constructs expressing engineered nucleases such as Transcription activator-like effector nucleases (TALEN), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, or Zinc-finger nucleases (ZFN).
The Mutation Detection Kit includes High-Fidelity DNA polymerase for amplifying the target regions from cells, and T7 Endonuclease I for recognizing and detecting the mismatches caused by gene editing tools. It provides an easy and reliable approach for estimating the efficiency of genome editing.
Applications
Detect the gene mutagenesis and SNP, for cleavage efficiency assay caused by ZFNs, TALENs, CRISPR/Cas9 or other gene editing tools.
Kit Components
High-Fidelity DNA polymerase: 25rxn: 10ul, 100rxn: 40ul 5X PCR Reaction Buffer: 25rxn: 150ul, 100rxn: 600ul T7 Endonuclease I: 25rxn: 25ul, 100rxn: 100ul 10X T7 Endonuclease I Reaction buffer: 25rxn: 50ul, 100rxn: 500ul Control Template DNA: 25rxn: 30ul, 100rxn: 120ul Control Primer Mix: 25rxn: 30ul, 100rxn: 120ul Protease K: 25rxn: 25ul, 100rxn: 100ul dNTP: 25rxn: 15ul, 100rxn: 60ul
Storage and Stability
Store all components at -20°C upon receipt.