Antigen identified by monoclonal antibody F8, AU, Auberger B antigen, B CAM cell surface glycoprotein, B cell adhesion molecule, B-CAM cell surface glycoprotein, Basal cell adhesion molecule (Lu and Au blood groups), Basal cell adhesion molecule (Lutheran blood group), Basal cell adhesion molecule, Basal cell adhesion molecule Lu and Au blood groups, Basal cell adhesion molecule Lutheran blood group, Bcam, BCAM_HUMAN, CD239, CD239 antigen, F8/G253 antigen, Glycoprotein 95kDa, LU, Lutheran, Lutheran antigen, Lutheran blood group (Auberger b antigen included), Lutheran blood group Auberger b antigen included, Lutheran blood group glycoprotein, MSK19
Laminin alpha-5 receptor. May mediate intracellular signaling.
Applications
Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:3000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human BCAM at C-terminal. Species sequence homology: porcine (91%), bovine (82%), equine (91%), sheep (91%), |canine (91%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human BCAM. Species Crossreactivity: mouse and rat
TDS
NCBI
UniProt
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