DnaK type molecular chaperone HSP70 1, Epididymis secretory protein Li 103, FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, Heat shock 70kD protein 1, Heat shock 70kD protein 1/2, Heat shock 70kD protein 1A/1B, Heat shock 70kD protein 1A, Heat shock 70kD protein 1B, Heat shock induced protein, HEL S 103, HSP70 1, HSP70 1B, HSP70 2, HSP70-1/HSP70-2, HSP70-1A, HSP70.1, HSP70.1/HSP70.2, HSP70I, HSP71_HUMAN, HSP72, HSPA1, HSPA1A, HSPA1B
The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity,
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide coupled to KLH.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes endogenous HSP70 proteins. Species Crossreactivity: Human, mouse and rat