AAG13, Aging associated gene 13 protein, Aging associated protein 13, Aging-associated gene 13 protein, Protein S100 A16, Protein S100-A16, Protein S100-F, Protein S100F, S100 calcium binding protein A16, S100 calcium-binding protein A16, S100A16, S100F, S10AG_HUMAN
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:100-200 Immunofluorescence: 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to S100A16. Species sequence homology: Porcine (90%), Bovine (90%), Equinse (90%), Sheep (90%), Canine (90%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total S100A16. Species Crossreactivity: Human, mouse and rat
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