Hsh 155, MDS, Pre mRNA processing 10, Pre mRNA splicing factor SF3b 155kD subunit, PRP 10, PRP10, PRPF 10, PRPF10, SAP 155, SAP155, SF3B 1, SF3b155, Spliceosome associated protein 155, Splicing factor 3b subunit 1 155kD, Splicing factor 3B subunit 1
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight. ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human SAP 155 at N-terminal. Species sequence homology: porcine (100%), bovine (100%), equine (100%), sheep (100%), canine (100%), chicken (100%), xenopus (100%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human SAP 155. Species Crossreactivity: mouse
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