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528159 Rabbit Anti-SETD8

Specifications
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q9NQR1
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
H4 K20 HMTase, H4 K20 specific histone methyltransferase, H4-K20-HMTase SETD8, Histone H4 K20 methyltransferase, Histone lysine N methyltransferase H4 lysine 20 specific, Histone-lysine N-methyltransferase SETD8, Lysine N-methyltransferase 5A, N-lysine methyltransferase SETD8, Pr SET 7, Pr SET7, PR-Set7, PR/SET domain containing protein 07, PR/SET domain containing protein 8, PR/SET domain-containing protein 07, PR/SET07, PrSET7, SET 07, SET 8, SET domain containing (lysine methyltransferase) 8, SET domain containing 8, SET domain containing lysine methyltransferase 8, SET domain containing protein 8, SET domain-containing protein 8, SET07, SET8, SETD 8, setd8, SETD8_HUMAN

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specificallymonomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tagfor epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role inthe silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance ofproper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.

Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human SETD8.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human SETD8. Species Crossreactivity: Mouse and rat
Conjugates
Pricing
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