35kD pulmonary surfactant associated protein, 35kD pulmonary surfactant-associated protein, Alveolar proteinosis protein, COLEC4, Collectin 4, Collectin-4, FLJ50593, FLJ51913, FLJ61144, FLJ77898,, FLJ79095, FLJ99559, MGC133365, MGC198590, OTTHUMP00000019928, OTTHUMP00000019929, OTTHUMP00000019930, OTTHUMP00000019931, PSAP, PSP A, PSP-A, PSPA, pulmonary surfactant -associated protein, 35KD, pulmonary surfactant apoprotein PSP-A, pulmonary surfactant associated protein A1, Pulmonary surfactant-associated protein A1, SFTA1_HUMAN, SFTP1, SFTPA1, SFTPA1B, SP A, SP A1, SP-A, SP-A1, SPA, SPA1, surfactant protein A1, surfactant protein A1 variant AB'D' 6A2, surfactant protein A1 variant AB'D' 6A3, surfactant protein A1 variant AB'D' 6A4, surfactant protein A1 variant ACD' 6A2, surfactant protein A1 variant ACD' 6A3, surfactant protein A1 variant ACD' 6A4, surfactant protein A1 variant AD' 6A, surfactant protein A1 variant AD' 6A2, surfactant protein A1 variant AD' 6A3, surfactant protein A1 variant AD' 6A4, surfactant protein A1B, surfactant, pulmonary associated protein A1A, surfactant, pulmonary associated protein A1B, surfactant-associated protein, pulmonary 1
This gene encodes a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens. Mutations in this gene are associated with idiopathic pulmonary fibrosis. Alternate splicing results in multiple transcript variants.
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human SFTPA1. Species sequence homology: Bovine (82%), Equine (90%), Sheep (82%), Rabbit (91%), Canine (91%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total SFTPA1. Species Crossreactivity: Human, mouse and rat