CMM6, DNA repair protein XRCC3, RAD51 like, X ray repair complementing defective repair in Chinese hamster, X ray repair complementing defective repair in Chinese hamster cells 3, X ray repair cross complementing protein 3, X-ray repair cross-complementing protein 3, XRCC 3, XRCC3, XRCC3_HUMAN
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Belongs to the recA family. RAD51 subfamily. Interacts with RAD51C and RAD51.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:3000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Immunofluorescence (IF-IC): 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human XRCC3.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human XRCC3.
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