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544477 Human Anti-SARS-CoV-2 Spike RBD

Specifications
Clone Type
Monoclonal
Host
Human
Source
Human
Swiss Prot
P0DTC2
Isotype
IgG1,Fc
Clone Number
A1
Grade
Affinity Purified
Applications
E N
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Spike glycoprotein comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). For many coronavirus (CoVs), S is cleaved at the boundary between the S1 and S2 subunits, which remain non-covalently bound in the prefusion conformation. The distal S1 subunit comprises the receptor-binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion machinery. S is further cleaved by host proteases at the so-called S2' site located immediately upstream of the fusion peptide in all CoVs. This cleavage has been proposed to activate the protein for membrane fusion via extensive irreversible conformational changes. However, different CoVs use distinct domains within the S1 subunit to recognize a variety of attachment and entry receptors, depending on the viral species. Endemic human coronaviruses OC43 and HKU1 attach via their S domain A to 5-N-acetyl-9-O-acetyl-sialosides found on glycoproteins and glycolipids at the host cell surface to enable entry into susceptible cells. MERS-CoV S uses domain A to recognize non-acetylated sialoside attachment receptors, which likely promote subsequent binding of domain B to the entry receptor, dipeptidyl-peptidase 4. SARS-CoV and several SARS-related coronaviruses (SARSr-CoV) interact directly with angiotensin-converting enzyme 2 (ACE2) via SB to enter target cells.

Applications
Suitable for use in GICA (Gold Immunochromatography Assay), ELISA and Neutralization. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000-1:100,000 Neurtalization: 1:100-1:10,000, the binding signal was inhibited by ACE2 protein-HRP conjugated inhibitor, with a 0.1074ug/ml IC50. GICA: 1:10,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa319-541 from human Novel Coronavirus Spike glycoprotein(S), expressed in 293 cells (HEK293).
Form
Supplied as a liquid in PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV). Does not react with MERS or SARS-CoV spike protein.
Conjugates
Pricing
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