COL4-A5; ASLN; ATS; CA54; Collagen Alpha-5(IV)chain; Alport Syndrome
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-2ug/ml Immunocytochemistry (formalin fixed cells): 5-20ug/ml Immunohistochemistry (formalin fixed frozen): 5-20ug/ml Immunohistochemistry (paraffin): 5-20ug/ml ELISA: 0.05-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Gly1461-Thr1685 from human Collagen Type IV Alpha 5, expressed in E.coli.
Form
Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Collagen Type IV Alpha 5.
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