Nanog, Nanog homeobox, 2410002E02Rik, ENK, ecat4, ES cell-associated protein 4, early embryo specific expression NK family, early embryo specific expression NK-type homeobox protein, homeobox protein NANOG, homeobox transcription factor Nanog
Applications
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:128,000 Western Blot: 0.5-2ug/ml. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa270-284, C-DLEVNLEATRESHAH, from mouse Nanog from the internal region.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse Nanog.
References
1. (Zbinden et al, EMBO J. 2010 Aug 4;29(15):2659-74. PMID: 20581802).