POU6F1 cDNA

Accession #

NP_002693.3

Shipping Temp

Blue Ice

Storage Temp

-20°C

BRN5, MPOU, TCFB1

POU6F1 belongs to the POu transcription factor family. This protein is transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3'). In the embryo, it is expressed exclusively in the developing brain, whereas in the adult its expression is restricted to brain, heart, skeletal muscle and lung. In the brain, the highest expression levels are found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.

Vector Description

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.

cDNA Size

906bp

Preparation before Usage

1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.

Nucleotide Sequence

ATGCCAGGGATCAGCAGTCAGATCCTCACCAATGCTCAGGGACAGGTTATTGGAACCCTTCCATGGGTAGTGAACTCAGCTAGTGTGGCGGCCCCAGCACCAGCCCAAAGCCTGCAGGTCCAGGCCGTGACCCCCCAGCTGTTGTTGAACGCCCAGGGCCAGGTGATTGCGACCCTGGCTAGCAGCCCCCTGCCTCCACCTGTGGCTGTCCGGAAGCCAAGCACACCTGAGTCCCCTGCTAAGAGTGAGGTGCAGCCCATCCAGCCCACACCAACCGTGCCCCAGCCTGCTGTGGTCATTGCCAGCCCAGCTCCAGCCGCCAAGCCATCTGCCTCTGCTCCTATCCCAATTACCTGCTCAGAGACCCCCACCGTCAGCCAGTTGGTGTCCAAGCCACATACTCCAAGTCTGGATGAGGATGGGATCAACTTAGAAGAGATCCGGGAGTTTGCCAAGAACTTTAAGATCCGGCGGCTCTCGCTGGGCCTTACACAGACCCAGGTGGGTCAGGCTCTGACTGCAACGGAAGGTCCAGCCTACAGCCAGTCAGCCATCTGCCGGTTCGAGAAGCTAGACATCACACCCAAGAGTGCCCAGAAGCTAAAGCCGGTGCTGGAAAAGTGGCTAAACGAAGCTGAACTGCGGAACCAGGAAGGCCAGCAGAACCTGATGGAGTTTGTGGGAGGCGAGCCCTCCAAGAAACGCAAACGCCGCACCTCCTTCACCCCCCAGGCCATAGAGGCTCTCAATGCCTATTTTGAGAAGAACCCACTGCCCACAGGCCAGGAGATCACTGAAATTGCTAAGGAGCTCAACTACGACCGTGAGGTAGTGCGGGTCTGGTTCTGCAATCGGCGCCAGACGCTCAAGAACACCAGCAAGCTGAACGTCTTTCAGATCCCTTAG

Translation Sequence

MPGISSQILT NAQGQVIGTL PWVVNSASVA APAPAQSLQV QAVTPQLLLN AQGQVIATLASSPLPPPVAV RKPSTPESPA KSEVQPIQPT PTVPQPAVVI ASPAPAAKPS ASAPIPITCSETPTVSQLVS KPHTPSLDED GINLEEIREF AKNFKIRRLS LGLTQTQVGQ ALTATEGPAYSQSAICRFEK LDITPKSAQK LKPVLEKWLN EAELRNQEGQ QNLMEFVGGE PSKKRKRRTSFTPQAIEALN AYFEKNPLPT GQEITEIAKE LNYDREVVRV WFCNRRQTLK NTSKLNVFQIP

Storage and Stability

Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Form

Supplied as a lyophilized powder.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a lyophilized powder.