Suitable for use in FLISA, Western Blot and Immunhistochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to DYKDDDDK, conjugated to KLH.
Form
Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes DYKDDDDK tag.
References
1. Valdivia CR et al. GPD1L links redox state to cardiac excitability by PKC-dependent phosphorylation of the sodium channel SCN5A. American Journal of Physiology - Heart and Circulatory Physiology. 2009;297(4):H1446-H1452. doi:10.1152/ajpheart.00513.2009.