Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:100-1:10,000 Optimal dilutions to be determined by the researcher.
Hybridoma
Spleen cells from immunized mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Canine immunoglobulin.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes canine IgG, IgA, IgM.
References
1. Tipold, A. et al. (2000) Presumed immune-mediated cerebellar granuloprival degeneration in the Coton de Tuléar breed. J Neuroimmunol. 110: 130-3, 2. Altug, N. et al. (2010) Immunostimulatory Effects of Aloe vera and &beta-Glucan on Cellular and Humoral Immune Responses Following Vaccination with Polyvalent Vaccines in Dogs. Kafkas Univ Vet Fak Derg 16: 405-12
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