Suitable for use in ELISA, Western Blot, Immunoprecipitation Immunoflourescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Western Blot: 1:250. Band representing ABC A1 is seen at ~220kD. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC A1 signal. Note: It is important not to boil the sample before loading onto a gel. Boiling can cause aggregation in large proteins, resulting in the protein’s inability to enter the gel. Post-nuclear lysates of 9-cis-retinoic acid and 22R-hydroxycholesterol induced raw macrophages were used for the positive control experiments. Immunoprecipitation: See Krimbou and/or Denis, et al. references. Immunohistochemistry: 1:100 Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Partial peptide corresponding to a sequence somewhere between amino acids 1100-1300 of the human ABCA1 gene.

Supplied as a liquid in Tris-citrate, pH 7.5, 0.02% sodium azide.

Purified by immunoaffinity chromatography.

Recognizes human ABC A1. Species Crossreactivity: mouse, rat and hamster.