Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of Actin have been identified, of these Actin beta is one of the three isoforms found in vertebrates. Defects in Actin beta are a cause of juvenile dystonia, a condition that results in movement disorders due to disordered muscle tonicity, abnormal posture, development malformations and sensory hearing loss.
Applications
Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysates
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to an 16aa sequence from near the amino terminus of human Actin beta.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ACTIN BETA (N-TERMINAL) at ~41.7kD. Species Crossreactivity: mouse and rat.